目的 通过对使用多效唑处理后的麦冬药材进行安全性和有效性综合评价，为麦冬生产中科学、合理的使用多效唑提供理论依据。方法 施用不同浓度多效唑处理麦冬植株，收集药材样品。从麦冬药材外观性状，黄酮、皂苷、多糖三大类有效成分含量各方面综合分析麦冬有效性;对多效唑残留量进行检测，参照“GB2763-2014”多效唑摄入限量和《中国药典》麦冬用量评价多效唑对麦冬药材安全性的影响。结果 多效唑对麦冬药材性状质量影响不显著;对麦冬多糖和黄酮含量均有不同程度的升高作用，对麦冬皂苷含量有一定的降低作用。多效唑按照3 kg·亩-1用量最高残留和麦冬最大用量计算，远低于每日允许摄入量(ADI:0.1 mg·kg-1×体重)。结论 在麦冬生产中可根据实际情况限量使用多效唑。
Abstract
OBJECTIVE To provide a theoretical basis for scientific and rational use of paclobutrazol in the production of Ophiopogonis Radix. METHODS Different concentrations of paclobutrazol were applied to Ophiopogonis Radix plants, and medicinal samples were colleted. The efficacy of Ophiopogonis Radix were comprehensively analyzed from the appearance of the herb and the contents of three kinds of effective components: flavonoids, saponins, and polysaccharides. Residues of paclobutrazol were detected. The effect of paclobutrazol use on the safety of Ophiopogonis Radix was evaluated according to the acceptable daily intake (ADI) of paclobutrazol in the GB2763-2014 and the amount of usage of paclobutrazol required by Chinese Pharmacopoeia. RESULTS Paclobutrazol had no significant effects on the Ophiopogonis Radix appearance; and the contents of polysaccharides and flavonoid were increased in varying degrees, and saponins content were decreased. The daily intake of paclobutrazol was far less than the ADI (0.1 mg·kg-1× body weight) when calculated using the maximum residue of paclobutrazol at the usage of 3 kg·acres-1 and the maximum usage amount of Ophiopogonis Radix in Chinese Pharmacopoeia. CONCLUSION Paclobutrazol can be used within limits according to the actual situation in Ophiopogonis Radix production.
关键词
麦冬 /
多效唑 /
有效性 /
安全性 /
黄酮 /
皂苷 /
多糖
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Key words
Ophiopogonis Radix /
paclobutrazol /
efficacy /
safety /
flavonoid /
saponins /
polysaccharide
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中图分类号：
R282
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参考文献
[1] JIA H T,DANG J D,LIU F L. Physiological function mechanism and application of multi-effect triazole . J Anhui Agric Sci(安徽农业科学),2003,31(2):323-324.
[2] LIU C L. World Pesticide(世界农药大全) . Beijing: Chemical Industry Press,2002:365-367.
[3] DU Y Q,CHEN R M. Influence of wort, paclobutrazol on tissue culture of barley . Acta Agric Shanghai(上海农业学报),1998,14(2):85.
[4] YU S,CHENG B,YUAN Z H. Residual dynamics of 15% paclobutrazol wettable powder on cabbage and soil . J Agro-Environ Sci (农业环境科学学报),2007,26(5):1768-1771.
[5] XIE Y L,ZHANG C H,LIANG Z Y,et al. Residue determination of paclobutrazol in litchi with capillary gas choromatography . Mod Agrochem(现代农药), 2011,10(1):36-37.
[6] Ch. P(2015) Vol Ⅰ(中国药典2015年版.一部) . 2015:155.
[7] ZHAN N,TAO S S,XIE M C. Effects of MET on growth, development and yield of dwarf lilyturf Ophiopogon japonicus(L. f. )Ker-Gawl . Hubei Agric Sci(湖北农业科学), 2014,53(12):2835-2837,2838.
[8] QIU J P. The reparative regeneration technology of soil that was contaminated by paclobutrazol. Acta Pedolog Sin (土壤学报),2002,39(1):45-51.
[9] LIN Q X,LI M,ZHOU H Y. Study of the influence of plant growth regulators on Ophiopogon japonicus′s total saponins and total polysaccharide content . Mod Chin Med(中国现代中药),2014,16(5):399-401,409.
LIN Q X. Study on the effects of plant growth regulators on the quality of Ophiopogon japonicus. Wenjiang:Chengdu University of Traditional Chinese Medicine,2014.
ZHAI Y Y,GUO B L,HUANG W H. Detection of agent“zhuanggenling” and investigation of utilization of plant growth retardants in traditional Chinese medicine cultivation. China J Chin Mater Med (中国中药杂志), 2015,40(3):414-420.
LAN H C. Experiment research of paclobutrazol applied in Liriopespicatavar prolifera . Wuhan:Huazhong University of Science and Technology,2010.
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脚注
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基金
国家科技部重大专项课题“重大新药创制”项目子课题(2014ZX09304307-002);科技部科技惠民项目(2013GS510102);四川省中医管理局科研项目(2014F038); 四川省科技厅科技支撑项目(2015SZ0033)
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